I am American learning Spanish on Duolingo.
Trying to understand the difference between vosotros (they all) and ellos/ellas/Ustedes (they all)
Is vosotros informal, whereas ellos/ellas/Ustedes is formal.
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Vosotros is the plural informal "you" in some countries. In other countries, instead of vosotros, people say "ustedes."
But in all countries, the plural formal "you" is "ustedes."
You should pick the regional variant you want to concentrate on learning, and stick consistently with that; and make sure that your learning materials match up with your target variant.
How do you conjugate a verb with "ustedes"? Exactly the same as for ellos/ellas.
That's why, when you read a conjugation table, you'll see ustedes listed in the same section of the table as ellos/ellas. But the meaning is different, as explained above.
Pablo y Carmela, ¿vosotros queréis acompañarnos? | Do you (plural, informal) want to come with us?
Pablo y Carmela, ¿ustedes quieren acompañarnos? | Do you (plural, informal) want to come with us?
Don Roberto, ¿usted y su esposa quieren acompañarnos? | Do you (plural, formal) want to come with us?
Manuel y sus hermanos quieren acompañarnos. | They want to come with us.
Note. If you want to communicate with people in or from Spain, learn vosotros. Otherwise you can skip it. For more details about vosotros regions, see Is Spain the only country that uses "vosotros" for "you all"?.
the confusion might arise from the fact that, meanwhile "usted/ustedes" is the formal pronunciation of the second person pronoun(you), is conjutated the same way as the third person(he/she/they) "el/ella,ellas/ellos".
so to begin i will explain how the second personal pronoun (you) works in Spanish
from the less formal to the most formal:
this is the less formal use, you would use it in casual conversation , can be exchanged with you. additionally, this pronoun is only used by regions and has been totally removed from some countries: reference : https://en.wikipedia.org/wiki/Voseo#Geographical_distribution, countries that have discarded this pronoun will use "Usted/Ustedes" instead, it has its own conjugations
Tu (doesn't have a plural form)
this is the most commonly accepted and used pronoun for the second person globally, it is also used for causal conversation but it adds a level of cordiality that you would use for people close to you, it has its own conjugation
highest level of formality, you would use it with people you respect, regions where the "vos" has been discarded will use this to address strangers with respect, it doesn't have its own conjugation, it will use the same conjugation as the third person (he/she/they) - el/ella, ellos/ellas